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Workstation
Live
Cell Imaging Systems
Contact & Support
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Leica AF6000
Advanced
fluorescence
imaging
system
New Dynamics
in Live Cell
Imaging
The Leica
AF6000 is a
fully
integrated
system for
advanced
fluorescence
imaging,
providing
solutions
that evolve
with your
changing
research
requirements.
From
overlaying
multi-channel
images to
acquiring
three
dimensional
and time
lapse data,
a wealth of
solutions
are included
as standard
for image
documentation,
quantification,
enhancement
and
analysis.
Designed to
completely
harmonize
microscope,
camera and
application,
the modular
AF6000
system is
available
for both
upright and
inverted
microscopes.
Additional
application
modules can
be added to
extend the
functionality
for
deconvolution
and 3D
visualization.
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High speed image
capture ensures minimum exposure of
your sample and effortless
acquisition of large
multi-dimensional data sets
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Multi channel
acquisition to generate overlays at
the click of a button
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Acquire 3D data sets
with an easy-to-use Z stacking
dialog
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Perform time lapse
experiments and generate AVI movies
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Mark and Find
capabilities for multi site
experiments
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Tile Scan feature for
automatic scanning, image capture
and merging of large areas to
provide a single panoramic view
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Full range of
enhancement tools for improving the
appearance of the image
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Measurement and
presentation features for image
quantification
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A range of advanced
deconvolution options
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3D Visualization
package: Display and interact with
3D data volumes
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A range of advanced
deconvolution options
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Display and interact
with 3D data volumes with the 3D
Visualize package
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Rich in features and possibilities
Software that caters to your needs
The simplicity of the software interface will give
you the confidence to push further. Designed with
the ergonomics of laboratory imaging in mind, it
guides you through set-up and analysis processes and
will have all members of your team up to speed in
record time.
See more
The system is available with either a single or dual
monitor display, offering a compact solution for
laboratories where space is at a premium or the
advantages of spreading your work over a lager area
for a more complete picture with two screens.
Total solution
A single source supply offers you a complete system
with highest functionality from a number of high
quality components.
Application Modules
LAS AF Deconvolution
LAS AF Deconvolution provides advanced
fluorescence imaging and offers sophisticated
deconvolution for 3D microscopic images. This
application module provides extremely high
resolution image restoration.
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Remove haze and blur by
reassigning light scattered by 3D
fluorescence imaging
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All necessary deconvolution
parameters automatically loaded from the
data set
-
Fully integrated
deconvolution algorithms for both blind
adaptive and non blind deconvolution, as
well as an inverse filter algorithm
Possibility to use a synthetic
point spread function, calculated from known
Leica objective data to deconvolve the data set
Colocalization
The colocalization tool allows measurement of
the measurement of Pearson’s correlation,
overlap coefficient, colocalization rate and
colocalization area. Regions of spatial overlap
can be easily identified by drawing regions of
interest into the cytofluorogram.
LAS AF 3D Visualization
LAS AF 3D Visualization enables
three-dimensional data sets to be viewed from
any angle. Your data can be rotated on screen to
move through the viewing angles you require and
a movie can be generated for play back of the
three dimensional view.
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3D rendering and dynamic user
interaction of volume
Compile multi-channel 3D movies
Unique TIRF solution
The unique Leica TIRF solution is fully
integrated into the AF6000 system series as an
additional contrasting method. At the heart of
the Leica AM TIRF solution lies a scanner, which
positions and rotates the laser beam within the
back-focal plane of the objective.
Highly integrated software control of the
scanner, allows the user to select different
angles for the laser at the slide-cell
interface. At the click of a button, the depth
of penetration of the evanescent field can be
changed for further elucidation of details in
the sample.
The scanner can rotate the laser to generate
different directions of the evanescent field.
A TIRF sensor detects the angle of the totally
reflected laser beam and intelligent software
algorithms confirm the presence and location of
detected structures within the evanescent field.
Benefits:
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Easy to use software with
auto alignment of laser and reproducible
control of depth of penetration
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Different directions of the
evanescent field
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Highly chromatic corrected
optics for brilliant image quality, without
color shift using different laser wavelength
Dynamic experimentation with
the Live Data Mode
You never know what will happen during the
course of a live cell experiment, but you know
that you should be prepared for anything. You
need full flexibility during your time lapse
experiment to change parameters in an instant.
The Leica Live Data Mode module allows you to
interactively monitor, log and change system
behavior, in response to what’s happening during
your experiment, for truly dynamic imaging.
The Live Data Mode is available as an additional
module to the Leica AF6000, but comes as
standard with the Leica AF6000 LX
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Make changes on the fly
The AF6000 Series Data Mode provides three
levels of direct user interaction. The first
level allows you to directly modify the
acquisition parameters during an active
experiment. With a single click, new
experimental setups are taken over and the image
acquisition commences with the new parameters.
This is crucial, for example, when time
intervals, exposure times or light intensities
have to be changed during a running experiment.
·
Respond to your changing
experiment
The second level enables users to define jobs
and activate them at any point during the course
of the experiment. A typical example could be
the addition of a DIC image to an experiment, at
any time, without interrupting the flow of the
active job. After acquiring the DIC image, the
experiment proceeds with the former experimental
setup. Consequently, a time lapse experiment can
consist of hundreds of fluorescence image stacks
but only a few single DIC images, as and when
they are required to document specific points in
the process.
Complexity with automation
The third level of interaction, allows you to
combine different jobs into a macro for
automated acquisition of an experiment with
varying experimental conditions throughout. A
single DIC image can be combined with a
fluorescence z-stack acquisition. For highly
sophisticated experiments, you may even combine
several macros together. The acquired images are
displayed online. During acquisition regions of
interest can be defined and intensity graphs
displayed and monitored.
Leica external filter wheels
The Leica external filter wheel provides an
extremely fast solution for live cell imaging
with the Leica DMI4000 B and Leica DMI6000 B
microscopes. It allows for fast switching
between different excitation or emission
wavelengths and high-speed regulation of light
intensity levels.
The Leica external filter wheels are fully
integrated into the AF6000 system series and can
easily be controlled within the LAS AF software
interface. The applied light source includes a
high speed shutter with 6 ms switching time. Its
120 W metal halogenide burner gives high
intensity levels, especially in the blue light
range.
Leica FRET SE Wizard
The Leica FRET SE module is an optional
application module available to add to the Leica
AF6000 series of widefield fluorescence systems.
Designed as a step-by-step wizard, you are
guided through the whole FRET experiment from
the definition of acquisition parameters, to the
identification of correction factors and
selection of FRET formulas for final calculation
of the FRET efficiencies.
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The Leica External Filter
wheels provide a dedicated solution for CFP/YFP
FRET with real time high speed acquisition
of up to 10 FRET triplets per second
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A non destructive FRET
method, ideally suited to experiments that
run over long periods of time
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A wizard approach guides the
user through each acquisition step to ensure
accurate results
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The wizard allows for the use
of reference samples for determination of
cross talk, background correction of FRET
images and determination of correction
factors by direct interaction with the image
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An online FRET image is
displayed during acquisition to allow
immediate feedback to the user
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FRET sensitized emission
formulas and FRET ratio formula are
available, for flexibility of analysis
results
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A FRET graph displays changes
in intensity
Store and recall of FRET
acquisition parameters and recall of FRET
corrections factors with a mouse click FRET
statistics can be easily exported into Excel
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